Dengue virus (DENV) in one of four serotypes is the cause of dengue fever. It is a mosquito-borne single positive-stranded RNA virus of the family Flaviviridae; genus Flavivirus.[1][2] All four serotypes can cause the full spectrum of disease.[1]
Its genome is about 11000 bases that codes for three structural proteins, capsid protein C, membrane protein M, envelope protein E; seven nonstructural proteins, NS1, NS2a, NS2b, NS3, NS4a, NS4b, NS5; and short non-coding regions on both the 5' and 3' ends.[1][3] Further classification of each serotype into genotypes often relates to the region where particular strains are commonly found or were first found.
The Dengue 1 virus appears to have evolved in the early 19th century Based on the analysis of the envelope protein there are at least four genotypes (1 to 4).
The rate of nucleotide substitution for this virus has been to be 6.5×10-4 per nucleotide per year, a rate similar to other RNA viruses.
The American African genotype has been estimated to have evolved between 1907 to 1949.
This period includes World War I and II which were associated with considerable movement of populations and environmental disturbance, factors known to promote the evolution of new vector borne viral species.
The primary life cycle of the dengue virus involves humans and mosquitoes.[5] There is also another cycle in Africa and also on Asia, which involves non human primates.[5]
The DENV E (envelope) protein, found on the viral surface, is important in the initial attachment of the viral particle to the host cell. Several molecules which interact with the viral E protein (ICAM3-grabbing non-integrin.,[6] CD209 ,[7] Rab 5 ,[8] GRP 78 ,[9] and The Mannose Receptor [10])have been shown to be important factors mediating attachment and viral entry
The DENV prM (membrane) protein, which is important in the formation and maturation of the viral particle, consists of seven antiparallel β-strands stabilized by three disulphide bonds
The glycoprotein shell of the mature DENV virion consists of 180 copies each of the E protein and M protein.
The immature virion starts out with the E and prM proteins forming 90 heterodimers that give a spiky exterior to the viral particle.
This immature viral particle buds into the endoplasmic reticulum and eventually travels via the secretory pathway to the Golgi apparatus. As the virion passes through the trans-Golgi Network (TGN) it is exposed to low pH. This acidic environment causes a conformational change in the E protein which disassociates it from the prM protein and causes it to form E homodimers.
These homodimers lie flat against the viral surface giving the maturing virion a smooth appearance. During this maturation pr peptide is cleaved from the M peptide by the host protease, furin.
The M protein then acts as a transmembrane protein under the E-protein shell of the mature virion. The pr peptide stays associated with the E protein until the viral particle is released into the extracellular environment. This pr peptide acts like a cap, covering the hydrophobic fusion loop of the E protein until the viral particle has exited the cell.[11]
Further information: Antibody-dependent enhancement and Dengue fever
The reason that some people suffer from more severe forms of dengue, such as dengue hemorrhagic fever, is multifactorial.
Different strains of viruses interacting with people with different immune backgrounds lead to a complex interaction. Among the possible causes are cross-serotypic immune response, through a mechanism known as antibody-dependent enhancement, which happens when a person who has been previously infected with dengue gets infected for the second, third or fourth time. The previous antibodies to the old strain of dengue virus now interfere with the immune response to the current strain, leading paradoxically to more virus entry and uptake.[12]
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